Sarah Sivilich

5 Questions 16 Answers 0 Followers

Questions related from Sarah Sivilich

What are your reasons for preferring external or internal threaded tubes for sample storage?

Which is better for minimizing cross-contamination and why? Is an o-ring essential for you and why?

12 December 2017 4,750 0 View

How can you make PCR specific to one allele?

Hi Friends, I have been having a nightmare genotyping these transgenic mice. What I have are heterozygous mice with a few knock-in point mutations. Originally, we used the same WT reverse primer...

11 November 2014 6,453 10 View

How can I get rid of false positives in PCR to be sure that I have a true heterozygous?

Hi friends, I have been working with a strain of transgenic mice with a mutated gene. The mutated region was used as the mutant forward primer. Therefore, anytime I get a PCR product, I am...

08 August 2014 4,502 8 View

What is the best antibody to detect ubiquitination for my protein MKP-1?

I would like to test for ubiquitination in my samples, but don't want to end up with a smeared blot. Can anyone recommend a good antibody to use? Or does any company make anti-ubiquitin MKP-1...

05 May 2014 1,537 1 View

Can a stripped blot still be used for phosphoprotein detection? Thanks!

PVDF membrane - only stripped once.

01 January 2014 4,102 5 View