Dear colleague Manaf Aldaoseri,

Have a nice time and good day.

Please follow the attached link you will find many answers you need.

i wish this is useful and help you.

Yours

A. El-Denglawey

https://www.researchgate.net/search.Search.html?type=question&query=how%20t%20prepare%20TEM%20sample%3F&tabViewId=59197acccbd5c27bf27ee3db&previous=researcher

Mr.A. Eldenglawey

thank you so much

Has Google banned you? :-)

The question is too broad. Probably, you have any concrete questions: preparation of TEM-grids, concentration of particles, sonic spraying trchnique, etc.

I would be glad to answer. 

Good luck!

Mr. Denis Korneev

thanks a lot. 

i have exactly some specific question , which is; what is the effect of different solutions (ethanol,distilled water,acetone,.....etc) where nano particles  are dispersed on the measurement.    

thank you again  

Dear Manaf,

There are a lot of good books and articles about TEM and sample preparation. Just google it. The effect of the solvent, obviously, is depend on the chemical nature of the particles and film on the TEM grid. For example, some solvents destroy Formvar film on the grid.

The preparation technique is depend on the properties of the sample too. There are few basic principles:

• Just incubate the grid (carbon or Formvar film covered) on a drop of solution and dry it out (usually, with a piece of filter paper);
• Sonic spraying technique;
• Negative staining for soft particles like viruses and VLP; 
• Ultrathin slices technique for bulk specimens.

They all are described in literature. 

Good luck!

Mr .Denis Korneev

thank you very much , it was useful tips.

excuse me , i have hollow silica nano-particles and i want to know the kind of solution which disperses in it  with retention of their structure.   

Don't worry, all is Ok :)

For the silica particles you can use a simple preparation technique:

1. Prepare a water suspension with concentration about 1 mg/ml. Shake it with Vortex or sonicate.

2. Drop a droplet (10 μ) on Parafilm surface and put a conventional TEM grid (e.g. Formvar film coated) at the droplet for 30 sec. Then just dry it out with a piece of filter paper and examine by TEM.

Good luck!