I have 5gm of valproic acid in which I have to use 7.2mg of the powder for my experiment. please suggest the process to store the remaining powder so that the shelf life of the powder should not be reduced. Please help me in this respect.
To increase shell life of powder , its air contact should be avoided.
We have ordered 3 different sets of primers for VDR (one is designed and two were from reference papers). At first condition, all the three didn't work out. So, to overcome this we have also...
10 June 2021 2,242 3 View
I have designed primers for vitamin D receptors using the IDT primer design tool. After that, I have checked its activity using the UCSC genome browser and Insilico PCR. The primers worked well...
07 April 2021 9,522 2 View
Hello everyone. I am trying to isolate RNA from 2 months old frozen brain samples by trizol method. After isolation and purification by kit, my yield was 3 micrograms from 15mg of tissue sample...
10 March 2021 2,308 7 View
I have pated 1 million cells on a coverslip in a 6 well dish to perform immunocytochemistry. Now I have to plate the same in a normal 6 well dish to study gene expression. Please suggest to me how...
30 July 2020 7,623 8 View
As a part of my study, I am supplementing Cholecalciferol (Vitamin D) to c57 mice. As per protocol, I have to give Vitamin D supplementation intraperitoneally. After supplementing Vitamin D,...
16 February 2020 3,716 11 View
01 February 2020 335 9 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...
20 July 2024 7,504 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
15 July 2024 2,414 1 View