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We have ordered 3 different sets of primers for VDR (one is designed and two were from reference papers). At first condition, all the three didn't work out. So, to overcome this we have also...
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I have designed primers for vitamin D receptors using the IDT primer design tool. After that, I have checked its activity using the UCSC genome browser and Insilico PCR. The primers worked well...
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Hello everyone. I am trying to isolate RNA from 2 months old frozen brain samples by trizol method. After isolation and purification by kit, my yield was 3 micrograms from 15mg of tissue sample...
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I have 5gm of valproic acid in which I have to use 7.2mg of the powder for my experiment. please suggest the process to store the remaining powder so that the shelf life of the powder should not...
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I have pated 1 million cells on a coverslip in a 6 well dish to perform immunocytochemistry. Now I have to plate the same in a normal 6 well dish to study gene expression. Please suggest to me how...
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As a part of my study, I am supplementing Cholecalciferol (Vitamin D) to c57 mice. As per protocol, I have to give Vitamin D supplementation intraperitoneally. After supplementing Vitamin D,...
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