11 December 2018 0 7K Report

What are the solutions to reduce the errors of the technical forces in the relay section at the posts of power transmission

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Why when I add Bromophenol Blue directly to my master mix, My PCR is inhibited?

Hi everybody, I have tried to make my home-made master mix for our laboratory. I have used two type of dyes , cresol red and Bromophenol Blue(BPB). I see when I use BPB my PCR is inhibited but no...

10 May 2024 7,492 8 View

Failed to merge meshes!?

Hi; Why is it possible to convert a part to a mesh part and replace it in an instance using the graphical interface in Abaqus, but when attempting to do the same using a script, an error...

01 May 2023 3,776 0 View

The good software or tool for TCR repertoire analysis (spectratyping) ?

Hey everybody, I am looking for an easy method and also software for analyzing TCR repertoires. is Spectratyping the easiest method? do you suggest any method which is easy to set it up in the...

24 April 2023 9,859 4 View

Multi-Particle Finite Element Model?

Hi there! I am presently engaged in compaction powder forming research, which involves working with a cubic representative volume element (RVE) comprising 1000 particles. In some particles, a...

13 April 2023 2,344 0 View

Multi-Particle Finite Element Model?

Hello I am currently working on implementing compaction powder forming using Abaqus. In order to do so, I need to select a representative volume element (RVE) from the entire model. However, some...

12 March 2023 9,631 0 View

Wulff Construction in crystals?

Why a sphere-like crystal would like to change to a Wulff crystal? I know that the equilibrium shape of a crystal is determined by the minimization of the surface energy and Wulff planes, but...

22 February 2023 3,330 6 View

Extracted huamn genome is partialy digested after treating with digestin enzymes?

Hi everyone, I have extracted the human genome from whole blood and tried to digest 2 ugs of it with 20 units of Bcll enzyme. unfortunately, after some trials, I observed that most of the genome...

27 December 2022 7,096 2 View

Question about valuaing data for a test reslut in spss?

hi dear, i ve developed a test where each item has a differnt reponse like a, b , c, d, how i should lable true scores in spss. should i transfer each score? thanks for your help. ?

25 December 2022 1,746 2 View

Must protein be denatured for Western blotting?

Hi everybody, Im about doing western blot and I've read that proteins must be denaturated for Western blotting. I question how it can be possible for antibodies to detect my specific protein if it...

16 December 2022 6,441 5 View

Why sometimes human transcripts are exactly same with negative strand??

Hi guys, I don't know why when I try to blast a segment of a human transcript sequence ( from the ensemble) on a gene (from NCBI), it sometimes returns the negative strand. But it has to be...

18 August 2022 3,856 0 View

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Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

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Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

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Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

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Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

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