Soil microorganisms, by actively participating in the decomposition and transformation of organic matter through diverse metabolic pathways, play a pivotal role in carbon cycling within soil systems and contribute to the stabilization of organic carbon, thereby influencing soil carbon storage and turnover. Soil microbes can break down plant organic matter to carbon dioxide or convert it to dissolved organic carbon (DOC) compounds. This leads either to long-term carbon storage, because DOC can bind to soil particles, or to the release of carbon back to the atmosphere as carbon dioxide. Microbes are critical in the process of breaking down and transforming dead organic material into forms that can be reused by other organisms. This is why the microbial enzyme systems involved are viewed as key 'engines' that drives the Earth's biogeochemical cycles. Microorganisms help return minerals and nutrients back to the environment so that the materials can then be used by other organisms. As the bacteria and fungi decompose. dead matter, they also respire. Plants constantly exchange carbon with the atmosphere. Plants absorb carbon dioxide during photosynthesis and much of this carbon dioxide is then stored in roots, permafrost, grasslands, and forests. Plants and the soil then release carbon dioxide when they decay. Soil microorganisms promote the decomposition of organic matter by secreting enzymes. The changes of biochar on soil enzyme activity are affected by the interaction between biochar, enzymes, and enzyme substrates. The active sites of biochar can absorb or desorb enzymes and their substrates. Soil organisms, including micro-organisms, use soil organic matter as food. As they break down the organic matter, any excess nutrients (N, P and S) are released into the soil in forms that plants can use. This release process is mineralization.