Does anyone know the amino acid sequence of rat NRF-2?

Hi, I'm trying to find a conjugated anti-rat Nrf2 monoclonal antibody for flow cytometry analysis in rat models. Unfortunately, i'm yet to find a conjugate that has been tested in rat samples. The...

03 March 2020 5,541 6 View

Is the weight of evidence (WOE) and the information value (IV), the most recommended method for the determination of predictor variables? Why?

I have a research project on the analysis of dengue cases vis a vis socio-demographic and physical attributes. I feel that there might be other methods that I can consider.

17 November 2018 7,412 1 View

Comparing means of items on a survey, between subjects or within?

If I administered a survey to participants where I had each participant rate 21 skills on their level of importance (using the same 5-point Likert scale) to a participant's job position, how...

28 August 2018 4,780 5 View

Is it necessary to synchronize cells in a cell cycle arrest assay?

I am currently planning to work on cell cycle arrest assays. I saw different literature have slightly different methods on cell synchronization. Some used serum free media, while some used 1%FBS,...

24 June 2018 4,922 2 View

What is the typical nitrogen consumption for a fume hood in an organic synthesis lab?

I'm setting up a new organic chemistry lab in an older building that does not have house nitrogen. Considering a generator instead of gas cylinders to supply the nitrogen gas needs for our...

23 April 2018 3,164 1 View

I am trying to look at dental composite in SEM and I want to see the 40nm fillers. Any advice on how to prepare the specimens?

I have tried fracturing the composite and looking at the fractured edge. I was wondering about burning out the resin or trying to dissolve it. Thanks!! -Nate

19 April 2018 6,635 2 View

How do I set up a restriction digestion reaction when my DNA concentration is very low?

I want to carry out a single RE digestion using Thermo scientific's NcoI. The reaction protocol requires a DNA concentration of 1ug/uL in a 20uL reaction. My DNA concentration is 1ug in 21.3ul....

13 November 2016 9,400 5 View

Lymphocyte Separation Medium vs ACK Lysing Buffer for removing red blood cells from pelleted Blood?

25 October 2016 3,693 3 View

Urgent: Ran out of TRIZOL for RNA ext. Is Buffer Saturated Phenol the same thing?

04 September 2016 4,393 3 View

Any advice on why KLF2 is not appearing on Western Blots?

We are trying to detect and quantify the presence of KLF2 which should appear as we have memory cells (CCR7+/CD62L+). We're using western blotting for that and we've tried both the traditional...

16 November 2015 6,247 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How to increase coupling range (distance between reader and sensor coils) in LC resonance circuit?

Hello great researchers, I am working on a wireless pressure monitoring project where sensing part is actually an LC resonance sensor and I am using a planar coil as a reader to get wireless...

11 February 2021 4,148 5 View

I am looking for a microbend optical sensor design...can anyone please provide me some idea about it??

I am working on a landslide model...i need an optical sensor to determine the amount of pore pressure and other values..i am thinking of using a microbend sensor. Can anyone please provide me with...

11 February 2021 5,911 2 View

How to choose the Hidden Layers Number and RBM Size in a DBN network?

Hello, When using a Deep Belief network (DBN) for images classification, how could we choose the number of hidden layers (number of Restricted Boltzmann Machines) and nodes (RBM size) in a hidden...

09 February 2021 4,618 5 View

Do there exist stand-alone permanently in-ground soil-moisture sensors with an installed GSM module, for outdoor use?

Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...

04 February 2021 2,047 4 View

How can I check how different treatments maintain a certain pattern?

Hi there, I have a network where I have used different aggregation methods for its nodes. Now I want to see if these aggregation methods changed their ranking of importance and their importance...

28 January 2021 5,513 3 View