What are the reason for non amplification of KASP markers during PCR?

More Milan Sapkota's questions See All

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

[SAP2000 related] I wonder why there is no any effect in the analysis result when I change the different parameters while modeling a frame structure?

(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...

06 October 2020 9,496 5 View

If a word file is shared with a person via Google Docs, can the recipient have access to the other files?

We can share and edit 'word' files online via Google Docs. It is an easy and convenient way of creating, sharing and editing the same document online between the concerned users. However, my...

22 September 2020 675 3 View

No title

I am working with new fish cell lines especially from cold-water fishes, so how can we choose the best program and process to get cells with higher viability and transfection efficiency?...

16 September 2020 9,574 2 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

Which gRNA designing tool for Crispr experiment can be effective for the gene knock out experiments in fish cell lines specially like Atlantic Salmon?

Any suggestions for critical things to be considered for the fish cell line as it is for cold water fish which is completely different than other mammalian cell lines.

14 August 2020 7,083 2 View

Values for Fouling rate in superheaters and economizers in biomass boilers?

I currently work on analyzing fouling rate in BFB biomass boiler, based on available measurements. Looking through literature, the only reported values I found were from certain TEMA Tables, from...

19 April 2020 1,973 5 View

Understanding the equation [C26.11-8, ASCE 7-16/SEI]?

While going through the ASCE 7-16, I encountered with the equation C26.11-8 under chapter 26 'Wind loads: General Requirements'. fn1=150/h (ft) ------------- Equation...

01 March 2020 1,643 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Jai Ghosh

Check the thermal cycle first. Then check whether the DNA was free from all impurities including RNA. Finally check the primers (possibly this seems to be the most probable culprit) and the buffers that you use during amplification.

Tulsani Nilam

Something is wrong but you have to find out problem systematically.

You can check with dilution of your primers and try to prepare new ones, hope it will work.

Also check the concentration of your DNA.

Hope it will help you.

Good Luck !

Milan Sapkota

Hello Andrew , i ran 96 DNA sample plate in KASP, of which 32 DNA sample were not amplified and showed 0al.el.ia in result. But other samples showed good result.