I am going to sequence some isolates that have some physiological and biochemical properties as Azotobacter spp. What primer or primers should I use to sequence those isolates ? Any suggestions are welcome.
Dear Amin
Please go through this link (http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300003).
Regards,
Sosanka
Thank you Sosanka for your link.
If you want to Identify the Azotobacter sp. strains, you can use 16 rRNA gene universal primers 27F and 1492R.
Thank you Shiva Krishna for your reference.
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