Number of diagnostics methods of malaria utilize biochemical testing (parasitic lactate dehydrogenase LDH), Fluorometrix method (Fluorochrome Hoechst 33458-AND of Plasmodium falciparum), or incorporation of precursor labeled radio (Hypoxanthine tritiated, actual gold standard in malaria drug research). This particular method is rarely used because it is expensive, required special infrastructure, and quite complex. In image segmentation process the gray level of a pixel belonging to an object is completely different from the background gray level pixels. So, thresholding becomes a simple but effective tool to separate those foreground objects from the background.
Papers:
1) Harijanto, Paul. Data dan Informasi Kesehatan, Epidemiologi Malaria di Indonesia. Kementrian Kesehatan Republik Indonesia. Jakarta. 2012.
2) Banimelhem, Omar, and Yahya Ahmed Yahya. "Multi-thresholding image segmentation using a genetic algorithm." In Proceedings of the International Conference on Image Processing, Computer Vision, and Pattern Recognition (IPCV), p. 1. The Steering Committee of The World Congress in Computer Science, Computer Engineering and Applied Computing (WorldComp), 2011.
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