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I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

How can we calculate theoretical stacking fault energy of unknown/new complex alloys?

Is there any method/theoretical approach to calculate the stacking fault energy of complex alloys such as high entropy alloys? The theoretical/ approximate value of SFE is needed for alloy design.

16 November 2020 7,074 3 View

Is the averaging plot of load-displacement graph is acceptable as the standard deviation of three samples of same specimen is high?

Hello everyone. I have three concrete prisms of the same size and obtained the load-displacement curve of three samples, but the standard deviation is 6.55..i plotted the average graph out of...

25 August 2020 3,968 1 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

15 June 2020 4,541 3 View

How COVID19 would affect the other research areas in Biology?

As the whole world is suffering with COVID19 infection; the research for vaccines are at its peak and so is the funding towards virology projects. Will it have a negative impact on funding in...

25 May 2020 8,579 3 View

How to make ROC plot for the validation of ligand-based-pharmacophore models in discovery studio?

Hello, I already made a ligand-based pharmacophore by utilizing 60 dataset compounds by catalyst module in discovery studio. But I want to explore the ROC curve of that pharmacophore in order to...

02 March 2020 9,044 5 View

How to solve the GROMACS error: The initial cell size (0.657461) is smaller than the cell size limit (0.683200)?

I am simulating a glycerol-water mixture system in a square box of length 3.5 nm. After the NPT step my box size decreased to 2.62984 nm. However, in the beginning of the production run step I...

27 February 2020 2,830 1 View

How to perform Scanning Electron Microscopy with liquid sample?

How the sample is prepared for SEM analysis in the case of a liquid sample?

05 January 2020 6,517 3 View

Can I import Abaqus simulated file to Ansys Workbench for further simulation?

I am using Abaqus/CAE for Laser Shock Processing (LSP), Can I import the simulated model file to Ansys Workbench for further analysis as I have to run two simulation for fatigue, FOD damage...

19 October 2019 7,031 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

Where can I find raw quantitative datasets on ecosystem services?

I'm an Undergraduate student on the search for a research topic which has to involve any ecosystem service(s) and a response to environmental change. I'm looking for raw quantitative data sets (I...

19 February 2021 5,911 2 View

Person-Codes for questionnaires in longitudinal studies: Is there an easy solution for better matching-rates?

Hi, I have a problem regarding matching of longitudinal data in my field of research (university students). We have a Person-Code for matching data measured in different time points, but 30%+ of...

17 February 2021 3,075 4 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

What are the available keys and descriptions for the identification of lice and fleas in domesticated animals?

I am an undergraduate student at the University of Peradeniya Sri Lanka, currently working on ecto-parasites in domesticated animals. I found difficulties in the identification due to a lack of a...

15 February 2021 277 1 View