Which methods are best for determining the inhibition zone for bacteria? agar well diffusion method or disk diffusion methods.
Hi Amiya Kumar Mandal
In my understanding, broth dilution is the best among the above-mentioned methods. It can provide a quantitative value of microbial susceptibility and ensure the proper mixing to microbes. But diluent for individual antimicrobial should be selected carefully that supports solubility well.
Disc diffusion and Etest have limitations on the diffusion capacity of antibiotics when their molecular sizes are large (eg. colistin).
Minimum inhibitory concentration (MIC) is the best method to evaluate the properties and susceptibility of antibacterials.
Disc diffusion method is best the because it could actually be use to determine the minimum inhibitory concentration of the antibacterial activities. You can estimate or measure the zone of inhibition. You have to know the size of the disc you are using.
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