How to increase the degree of labeling in amine and NHS ester conjugation?

I am trying to conjugate oligo modified in the center with amine to NHS-ester modified dye. As oligo is modified in the center of the chain, the degree of labeling achieved is less than 40...

10 November 2018 1,458 3 View

Do copper nanoparticles show shape and size dependent color variation ?

I read many research acticle regarding gold and silver nanoparticles have color variation as the size of particle changes. I am curious to know that does copper nanoparticles have similar...

09 October 2015 5,190 5 View

What is the reason for the presence of three plot ( for Exx, Eyy and Ezz) in dielectric constant vs photon energy plot for 2D structure?

While calculating the optical spectrum for nanowire (theoretically) I got three curves for all three different direction but as per my knowledge the spectrum contains two curve, one for z axis and...

31 December 2014 4,661 1 View

Is there any relation between the polarization of light and scattering of light?

During the study of optical spectrum for 2 D material, this question comes in my mind

31 December 2014 8,648 11 View

How to do passivation of nanowire in AtK virtual nanolab 11.8.2?

31 December 2014 2,481 0 View

If we made a nanowire by using semiconducting material like ZnTe or ZnO , are there electronic properties that will change or remain the same?

Electronic properties of nanowire

11 December 2014 921 17 View

Please explain why we need high quantum yield of nanoparticles?

I want to understand the factors affected by the quantum yield.

05 June 2014 3,113 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View