We are working on the morphological and molecular characterization of potential clones grapevine variety Vranac, the dominant black wine variety from Montenegro.
We analyzed 98 potential clones-vines based on qualitative and quantitative characteristics. During the two-year period, the analysis was conducted and 21 potential clones were identified. We tested the ELISA test using the BIOREBA AG reagent (http://www.bioreba.ch) for the presence of GLRaV-1, GLRaV-3, and GFLV, in the absorption readings at 405 nm. In all samples, presence was established.
We are interested in how to proceed. What methods can be applied to selected potential clones to remove these viruses?
We're getting ready beforehand.
Prof. Dr Branislava Sivčev
University of Belgrade
Faculty of Agriculture
Institute of Horticulture
Chair of Viticulture
There are methods for elimination of virus in plants. There is a chapter on this topic in the following books:
Plant Tissue Culture: Theory and Practice, A Revised Edition. 1996. Elsevier, Amsterdam
Introduction to Plant Tissue Culture (Second Edition). 2002. Science Publishers , Enfield, USA.
I am sure your Institute Library will have one of these books.
The two most commonly used methods to eliminate viruses in grapevine are thermotherapy (35°C for 3 months) and apex micrografting.
These links might be useful :
https://link.springer.com/chapter/10.1007/978-94-015-8951-2_26
https://www.youtube.com/watch?v=ghAFM1cDdd0&list=PL1dRoyJUp7dzFwFerYtHM6tUZzJlE8n9F&index=17
The French Institute for Vine and Wine (IFV) offers a virus clearing service through micrografting and I invite you to get in touch with the person in charge of the laboratory ([email protected]) if you wish to obtain further information.
Good luck !
Dear Branislava Vojislav Sivcev
Pls. find the attached files
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000200404
Hoping this will be helpful
Regards
Prof. Houda Kawas
https://books.google.com/books?id=emk1k1baiIkC&pg=PA13&lpg=PA13&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=y7m6kVPTY2&sig=KoERSmjfuEWFoxY1C9qGO8CtgXQ&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIQDAE#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false
http://www.fao.org/fileadmin/templates/agphome/documents/PGR/PubPGR/FAOIPGRI_list.pdf
https://books.google.com/books?id=0U8_AAAAQBAJ&pg=PA320&lpg=PA320&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=_DpGIjtjkv&sig=Uo8F9FyaUtssxV8ks37DuMfyvVk&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIRTAF#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false
https://books.google.com/books?id=SFuzZ1pWpxYC&pg=PA79&lpg=PA79&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=fesVq1S8Qa&sig=9jrq_l653fow21v4ZFQEJqk094E&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEITjAH#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false
https://books.google.co.in/books/about/FAO_IBPGR_Technical_Guidelines_for_the_S.html?id=emk1k1baiIkC
https://books.google.com/books?id=drLF8tKEWskC&pg=PT153&lpg=PT153&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=HKfbttoxtq&sig=xFIKtzA-7BUg1ko5RQN4wNA1lAI&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIWTAJ#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false
http://www.bioversityinternational.org/e-library/publications/detail/faoipgri-technical-guidelines-for-the-safe-movement-of-small-fruit-germplasm/
http://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=12989
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627144/
http://oeno-one.eu/article/view/1653
Many thanks to all participants who help me with their knowledge and experience in solving this problem
Branislava
Hi Branislava
Some references 1
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQFggmMAA&url=http%3A%2F%2Foeno-one.eu%2Farticle%2Fview%2F1653&usg=AOvVaw2MXgprd97cLuSBe8wUxqU_
2
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQFgg1MAM&url=http%3A%2F%2Fwww.avia.org.au%2Fpdf%2FVirusTestedClonesGWRDCFinalReport.pdf&usg=AOvVaw2Iz7LSmyS6oAC-TmuWlVJp
3
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQFgg6MAQ&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fjph.12314%2Fabstract&usg=AOvVaw0rK0H54YhL6gs80cvZVn9G
4
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQFghCMAU&url=http%3A%2F%2Fwww.graftedvines.com.au%2Fimages%2FGrapevine%2520Clones%25202006.pdf&usg=AOvVaw1I_2Sj5ts2BJjLm7zhKDyF
5
https://books.google.iq/books?id=EjwrDwAAQBAJ&pg=PA329&lpg=PA329&dq=selected+clones+grapevine,+remove+viruses&source=bl&ots=eYnR3oULpv&sig=CCH4vNKYC13OJjyqhfhY1ewkMNY&hl=en&sa=X&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQ6AEIRzAG
6
https://books.google.iq/books?id=G1IVY1kbUiwC&pg=PA47&lpg=PA47&dq=selected+clones+grapevine,+remove+viruses&source=bl&ots=EvaG1Odyxt&sig=6IDDlk9OxlIMPov5WEDFQxQbsXk&hl=en&sa=X&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQ6AEISTAH
7
https://books.google.iq/books?id=fkSxg-lBtO8C&pg=PA998&lpg=PA998&dq=selected+clones+grapevine,+remove+viruses&source=bl&ots=V-8iDoXwkq&sig=yV2MwFh6mzCb8rrZoshlo6WZbZE&hl=en&sa=X&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQ6AEITDAI
8
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&cad=rja&uact=8&ved=0ahUKEwiYgOmluqvXAhXB8qQKHQKyCGIQFghOMAk&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4661162%2F&usg=AOvVaw3AWPeNgos42HiHWZk6NQA2
9
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=11&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCCUwAA&url=http%3A%2F%2Fwww.graftedvines.com.au%2Fimages%2FGrapevine%2520Clones%25202006.pdf&usg=AOvVaw1I_2Sj5ts2BJjLm7zhKDyF
10
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCCowAQ&url=http%3A%2F%2Fiv.ucdavis.edu%2Ffiles%2F24346.pdf&usg=AOvVaw0qW-6Vo7tJry1fLLkuNi5D
11
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCDAwAg&url=http%3A%2F%2Fiv.ucdavis.edu%2Ffiles%2F108857.pdf&usg=AOvVaw0zbdZw0N0krbKfmhyEdB4D
12
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCDgwAw&url=http%3A%2F%2Fwww.scielo.br%2Fscielo.php%3Fscript%3Dsci_arttext%26pid%3DS0100-29452016000200404&usg=AOvVaw2I4TNyN_x7CfbQ8MTPFM7g
14
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCEkwBQ&url=http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2F0378111988903137%2Fpdf%3Fmd5%3D4f060190a958780733240e1e824c7a1d%26pid%3D1-s2.0-0378111988903137-main.pdf&usg=AOvVaw24soekPatTlq-YZMAx2tlz
16
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwiWsYafvavXAhWHh7QKHV2KA104ChAWCFcwCA&url=https%3A%2F%2Fvirologyj.biomedcentral.com%2Farticles%2F10.1186%2F1743-422X-11-186&usg=AOvVaw19eOTHCklXfXYa2dJ8ECAc
17
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=23&cad=rja&uact=8&ved=0ahUKEwiNlO2wwKvXAhUNZFAKHcZXBV44FBAWCDMwAg&url=https%3A%2F%2Fapsjournals.apsnet.org%2Fdoi%2Fpdf%2F10.1094%2FPHYTO-06-13-0179-R&usg=AOvVaw1-bi9UEdI_UqvUca_aIL6G
18
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=24&cad=rja&uact=8&ved=0ahUKEwiNlO2wwKvXAhUNZFAKHcZXBV44FBAWCDswAw&url=https%3A%2F%2Fapsjournals.apsnet.org%2Fdoi%2Ffull%2F10.1094%2FPHYTO-01-16-0035-R&usg=AOvVaw3fle1EhijPpzq-c-Gy0yxt
19
https://books.google.iq/books?id=-ePLBQAAQBAJ&pg=PA906&lpg=PA906&dq=selected+clones+grapevine,+remove+viruses&source=bl&ots=n1hmv-CzbE&sig=JMp4dFkPNaBQtS5-He0UQu6OWMs&hl=en&sa=X&ved=0ahUKEwiNlO2wwKvXAhUNZFAKHcZXBV44FBDoAQhAMAQ
20
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=26&cad=rja&uact=8&ved=0ahUKEwiNlO2wwKvXAhUNZFAKHcZXBV44FBAWCEIwBQ&url=http%3A%2F%2Fwww.wineland.co.za%2Fa-guide-to-grapevine-abnormalities-in-south-africa-virus-and-virus-like-diseases-shiraz-decline-part-4-5%2F&usg=AOvVaw21bwqWSKvZ_IJHRXtAVKub
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