What are the methods for clearing the potential clones from the virus?

Branislava Vojislav Sivcev @Branislava-Sivcev

13 July 2017 24 4K Report

We are working on the morphological and molecular characterization of potential clones grapevine variety Vranac, the dominant black wine variety from Montenegro.

We analyzed 98 potential clones-vines based on qualitative and quantitative characteristics. During the two-year period, the analysis was conducted and 21 potential clones were identified. We tested the ELISA test using the BIOREBA AG reagent (http://www.bioreba.ch) for the presence of GLRaV-1, GLRaV-3, and GFLV, in the absorption readings at 405 nm. In all samples, presence was established.

We are interested in how to proceed. What methods can be applied to selected potential clones to remove these viruses?

We're getting ready beforehand.

Prof. Dr Branislava Sivčev

University of Belgrade

Faculty of Agriculture

Institute of Horticulture

Chair of Viticulture

M. K. Razdan

There are methods for elimination of virus in plants. There is a chapter on this topic in the following books:

Plant Tissue Culture: Theory and Practice, A Revised Edition. 1996. Elsevier, Amsterdam

Introduction to Plant Tissue Culture (Second Edition). 2002. Science Publishers , Enfield, USA.

I am sure your Institute Library will have one of these books.

Olivier Geffroy

The two most commonly used methods to eliminate viruses in grapevine are thermotherapy (35°C for 3 months) and apex micrografting.

These links might be useful :

https://link.springer.com/chapter/10.1007/978-94-015-8951-2_26

https://www.youtube.com/watch?v=ghAFM1cDdd0&list=PL1dRoyJUp7dzFwFerYtHM6tUZzJlE8n9F&index=17

The French Institute for Vine and Wine (IFV) offers a virus clearing service through micrografting and I invite you to get in touch with the person in charge of the laboratory ([email protected]) if you wish to obtain further information.

Good luck !

Houda Kawas

Dear Branislava Vojislav Sivcev

Pls. find the attached files

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000200404

Hoping this will be helpful

Regards

Prof. Houda Kawas

https://books.google.com/books?id=emk1k1baiIkC&pg=PA13&lpg=PA13&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=y7m6kVPTY2&sig=KoERSmjfuEWFoxY1C9qGO8CtgXQ&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIQDAE#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false

http://www.fao.org/fileadmin/templates/agphome/documents/PGR/PubPGR/FAOIPGRI_list.pdf

https://books.google.com/books?id=0U8_AAAAQBAJ&pg=PA320&lpg=PA320&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=_DpGIjtjkv&sig=Uo8F9FyaUtssxV8ks37DuMfyvVk&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIRTAF#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false

https://books.google.com/books?id=SFuzZ1pWpxYC&pg=PA79&lpg=PA79&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=fesVq1S8Qa&sig=9jrq_l653fow21v4ZFQEJqk094E&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEITjAH#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false

https://books.google.co.in/books/about/FAO_IBPGR_Technical_Guidelines_for_the_S.html?id=emk1k1baiIkC

https://books.google.com/books?id=drLF8tKEWskC&pg=PT153&lpg=PT153&dq=grapevigne+virus%2BFAO/IPGRI&source=bl&ots=HKfbttoxtq&sig=xFIKtzA-7BUg1ko5RQN4wNA1lAI&hl=ar&sa=X&ved=0ahUKEwiYhpTyhJPVAhVjLZoKHahVDaIQ6AEIWTAJ#v=onepage&q=grapevigne%20virus%2BFAO%2FIPGRI&f=false

http://www.bioversityinternational.org/e-library/publications/detail/faoipgri-technical-guidelines-for-the-safe-movement-of-small-fruit-germplasm/

http://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=12989

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627144/

http://oeno-one.eu/article/view/1653

Branislava Vojislav Sivcev

Many thanks to all participants who help me with their knowledge and experience in solving this problem

Branislava

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