What is the effect of repeating annealing process on the same material? Does it softens the material more?

If we do annealing more than one time on the material consequently, does it soften the material more? or what?

31 December 2018 6,172 12 View

What are the values for Al 6061 in LS-Dyna card "MAT_ADD_EROSION"?

What are the values for 1. Maximum pressure at failure 2. Minimum principal strain at failure 3. Maximum effective strain at failure 4. Volumetric strain at failure 5. Number of failed integration...

31 December 2018 1,294 1 View

What is the "Secondary Crack Defect" ??

Secondary crack is observed in the sheared edge. What is significance of this secondary crack and why it is generated?

08 September 2018 1,859 0 View

What is the meaning of time period in step module of Abaqus Explicit? and How to decide the time increment in Abaqus Explicit?

I am trying to simulate punching of tube process in Abaqus. Speed of punch is 10 mm/min and it have to travel total 7 mm including the thickness of tube (1.5 mm). How to simulate the Process?...

07 August 2018 6,671 12 View

How to Fix "Solver error - time step too small" error in Ansys Explicit Dynamics?

Hello Everyone, I am trying to stimulate high speed impact punching process and after refining the mesh I encounter with this error. For refining the mesh at required region i used Sphere of...

05 June 2018 4,772 2 View

How high strain rate forming processes reduces spring back?

High strain rate forming processes, Flow stress, Spring Back

04 May 2018 655 8 View

What is the Johnson-Cook properties for HT9 steel?

Can anyone please tell me the Johnson-Cook properties for HT9 steel.

03 April 2017 9,044 2 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View