I am getting three to four absorption band while three fluorescence bands in some of compounds. what are the main characteristics information we can extract from spectra.
I agree fully with the excellent answer of Gert. Your question is too, too vague!! Why?
- you must give infos about your compounds. (e.g. purity (dryness, elemental analysis, NMR, IR...)
- you must give infos about your spectrophometer (e.g. single beam, dual beam or diodearrays.)
- you must give infos about your solvents (purity e.g. spectranal...) and the solvent cut-off wavelenghts.
- It seems you mean one maximum and more shoulders for every compound. If not, you have a mixture or your compounds are not pure. In this case, you cannot work in a scientific manner.
-you must give infos about your cuvettes (Quartz or plastic) for UV-Vis and fluorescence measurements.
- Please read the articles in Wikipedia about absorption and fluorescence.
-If you draw and see your molecule on paper or PC , you can find the UV absorption bands for typical chromophores (aromatic e.g. conjugated systems).
- For nonconjugated systems, the bands may be inaccessible for conventional spectrophotometers.
- Important!! Please read the article about Stokes Shift in the following link:
-https://en.wikipedia.org/wiki/Stokes_shift
JRG
Thanks for your inputs Sir. The peaks do not have shoulder, the bands are clearly distinguishable. i wil attached snapshot lator
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