Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What metal thin film houses also do annealing?

Could anyone provide names of metal thin film deposition vendors that also do high temperature annealing, in the range of 300-500 C?

27 December 2020 6,137 3 View

Vacuum freeze drying protocol in human skeletal muscle tissue methods, anyone?

I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the...

21 November 2020 1,423 1 View

Does anyone freeze samples in GeneJET RNA extraction kit lysis buffer?

For the GeneJET RNA purification kit from Thermo Scientific, does anyone know if you can lyse your sample (suspension cells in my case) in the lysis buffer from the kit and then keep the sample at...

18 October 2020 4,993 3 View

Looking for good/reliable betaine assay kits. Does anyone have experience with such a thing?

I am looking to quantify both human serum and skeletal muscle homogenate betaine concentrations via ELISA or colorimetric assay kits. Has anyone had a good experience with any products...

24 September 2020 4,550 3 View

Accurate curvature estimation of CT-scans (image stack)?

We have multiple CT-scans in the format of TIF image stacks. To get the mean curvature we first transform the stack to a triangular mesh (using Marching Cubes). To make the triangles in this mesh...

03 August 2020 7,921 4 View

Input from experts in environmental survey sampling design on draft sampling design decision tree?

Hi. I am trying to help provide some rough advice for selecting amongst alternative sampling designs to measure bird density or abundance. The surveys are short-term in duration such as for impact...

22 January 2020 2,978 3 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

Use Betas Estimated in Original Jones Model in Modified Jones Model STATA?

Hello, I can use the following codes in Stata to generate regression according to original Jones model. gen Jones1991_DA=. forval y = 2010(1) 2015{ forval i = 1(1) 6{ display `i' display `y'...

14 October 2019 6,438 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

How do you obtain ASI (Annual Survey of Industries) plant identifiers, to create a panel data?

Dear all, Is there anybody who has worked with ASI (Annual Survey of Industries) Firm-Level data? I have extracted the data files with STATA, and now I need help with creating a panel for...

11 February 2021 876 4 View

Where can I find FDI data by sector/industry for Asian countries like India and China for the last 30 years?

Common databanks like UNCTAD, OECD, WorldBank and ASEAN Website did not have the data for the last 30 years.

09 February 2021 4,701 4 View

What could be an interesting topic for a bachelor's thesis in a finance/marketing/computer science field?

Hi everyone! I would like to write my bachelor's thesis on a topic that's currently relevant in the sphere of finance, marketing or computer science (or if it's possible a topic concerning all the...

08 February 2021 1,300 12 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View