What are the different metabolites to be evaluated in Xanthosoma sagittifolium?

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

Why some samples don't give any peaks in HPLC when the rest does?

Hello everyone, Last week we ran an HPLC assay with bacterial supernatant samples. The metabolites measured were SCFA and I used a dilution factor of 2 for some samples and 7 for others (because...

22 January 2021 8,667 6 View

What is the role of pO2 and pCO2 in cell culture?

Using a Nova bioprofile (https://www.fannin.eu/wp-content/uploads/2018/07/BioProfile-Flex.pdf) to sample my CHO culture daily I get various metabolite results and also pO2 and pCO2 results. I am...

13 December 2020 7,998 1 View

How to identify possible metabolite from chromatogram?

We have done the analysis for a pre-clinical study and we have observed additional peaks in the chromatograms. We think that these peaks are metabolites. The concentration of the additional peaks...

02 December 2020 6,134 10 View

What is an efficient way of extracting metabolites for fungal isolate from soil for test on its antimicrobial activity?

Hi, I'm finding a method to extract metabolites to evaluate its antimicrobial activity but the identification of the fungi is not done yet. I'm looking for a universal way to extract the...

24 November 2020 2,273 3 View

What is the significance of serotonin and its metabolite on stress ?

What is the significance of increasing serotonin level of stressed rat compared to normal one and slight increase of 5HIAA level in stressed rat. With higher 5HIAA/5HT ratio in normal one.

21 October 2020 2,165 5 View

How to find compounds that are similar to others in term of structure and activity?

Dear all, I Have found in silico two compounds that are interesting for me, one is spermidine (a metabolite) and another one is negamycin. Negamycin has apparently an effect on my cellular models...

20 October 2020 8,394 3 View

Can anyone suggest me any article which contain specific plant metabolites against antimicrobial activity?

I would like to any lab based plant metabolites which directly interact with multidrug resistant bacteria or any wet lab based molecules able to do the same.

30 August 2020 2,629 4 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View

Tiki Antoine Marie Kevin

Good morning my dear!!

the different metabolites to evaluate in Xanthosoma sagittifolium are: sugar, protein, amino acids, phenolic compounds, flavonoids …

It depends on the focus of your research! What you want to demonstrate!! But all the primary and secondary metabolites present on your plant can be evaluated...

Fossong NENGI Abigaelle

Thank you,what about phytohormones and terpenoids?.