What are the correct coordinates to target RTN nucleus?

01 January 2019 0 2K Report

To target retrotrapezoid nucleus (RTN) in adult Wistar rats for drug micro-injections via steel cannula what are the coordinates with respect from Bregma (AP, DV and ML). Different authors have used different coordinates for RTN. I refered to Paxinos and Watson atlas (2005) but I was not sure because trapezoid body has the maximum extension just -9.7 mm from bregma and 10.5mm ventral to skull surface.

How much is RTN ventral to the Trapezoid body or parafacial nucleus?

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