Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

How to extract polygons of species distribution from books?

If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...

20 June 2020 4,391 1 View

To which family and genus belong this centipede?

Of this type of centipede (two centimeters long) there are many in my garden, located at 2,350 meters above sea level in the Andes of northern Peru (Chachapoyas, Amazonas department). Any idea to...

28 March 2020 6,277 7 View

Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

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20 January 2020 6,200 5 View

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Dear researchers, We are working in Colombia with the biological groups: primates and bats. We have been debating about landscape extent area/lenght, and also we have had read many articles about...

09 October 2019 5,771 3 View

Could exist a evidence of Regional Metamorphism in igneous rocks of Coasthal Batholith (Cretaceus) in Center of Peru?

Recently in a project, using microscopy and XRD studies, we identified the presence of actinolite (from ferromagnesians) and clinochlore (from biotites) in igneous rocks of Coasthal Batholith...

09 October 2019 6,451 2 View

Does noise affect, or compound, mercury poisoning in the human body relative to small-scale gold mining?

On-the-ground experience would suggest that sound/noise pollution and mercury contamination may be coincidental in artisanal and small-scale gold-mining stakeholders and their bodies. If so, does...

07 August 2019 5,302 1 View

Does noise affect, or compound, mercury poisoning in the human body relative to small-scale gold mining?

On-the-ground experience would suggest that sound/noise pollution and mercury contamination may be coincidental in artisanal and small-scale gold-mining stakeholders and their bodies. If so, does...

31 July 2019 5,350 1 View