Please Suggest me the best media for the growth of gram negative bacteria, mainly for :
E. Coli , Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa.
There are many agar media using for growth G-ve bacteria like MacConkey agar with crystal violet ,and there is some media used to differentiate genus from other like EMB to differentiate E.coli from other bacteriaceae, Milk agar to differentiate P.aeruginosa.
These bacteria can grow on many different media so what are you looking for?
With best regard dear Enass Ghassan Sweedan it is completely wrong that EMB can used for differentiation.
Alireza Mordadi I want to know the one media on which all these bacteria grow. your kind answer will be appreciated.
thanks
Dear Alireza
Why it's wrong for differentiate?
I said can use EMB differential for E.coli because its grow with green metallic sheen .can you feel me why this wrong?
Dear Enass Ghassan Sweedan
Because EMB it is not a differential medium and we have many E.coli bacteria do not growing such as you described.
Dear Alireza Mordadi can you send me any research article in which they used chocolate agar for the growth of these bacterias i mention.
its urgent please
Take it that we do not use chocolate agar to grow those bacteria.
We choose our medium according to samples and for example we use chocolate agar for detection Neisseria meningitidis suspected samples, so I do not think any research mentioned use of chocolate agar for those bacteria.
But those bacteria can also grow on chocolate agar.
I think chromoagar media batter than others for detection those bacteria..Fast and accurate
Do you want a selective media for each species of Gram Negative Bacteria cited or a selective medium for all the negative grams mentioned? I propose chromoagar media and MacKonkey agar, they are selective and easy to differentiate for species cited.
If you know a chromoagar, it is able to differ enterobacteriaceae families and Pseudomonas families at same time, pleas let me know because I have not studied about such media for about 2 years.
Regards
I checked addressed by @Banfitebiyi Gambogou , but I did not find my answer.
Merlino, J., Siarakas, S., Robertson, G. J., Funnell, G. R., Gottlieb, T., & Bradbury, R. (1996). Evaluation of CHROMagar Orientation for differentiation and presumptive identification of gram-negative bacilli and Enterococcus species. Journal of clinical microbiology, 34(7), 1788-1793.
Thanks.
I took just they do not sure in their article and I want to know there is a commercial medium or not?
Yes see this with reference. CHROMagar™ Orientation, Ref: RT410 / RT412
i think the agar only not enough for diferantiation you must do biochimical test
gram negative bacteria, (E. Coli , Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa.) macconkey agar is best as E. Coli (bright pink) , Klebsiella pneumoniae (pink mucoid colonies and large) , Acinetobacter baumannii pale colonies and Pseudomonas aeruginosa.pale with green pigmentation and oxidase positive ) you must
Hello Asad Ullah,
Your question seems simple but on the contrary, it requires a reflection not to waste agar.
Macconkey is the best agar for selecting gram-negative bacteria among others. To identify it, use ChromAgar media.
If you merely want a standard medium for growth, then really any type of rich medium would work fine for those organisms, such at nutrient agar, LB agar, TYE, YT, Luria etc. All the organisms you list will grow fine on these. If you want selective medium so that some grow and some don't, or you want to be able to differentiate between them, then the above answers are useful for various selective and diagnostic medium.
From experience:
They all grow well on LB-Agar, Nutrient agar.
Pretty sure they will also grow on M9, Muller-Hinton and Tryptic soy agars.
Good luck!
Hi,
I second the suggestion given by Prof. Michael. If it is just to culture them on plates, you may do so using any nutrient rich-solid media. If you are planning to isolate them specifically, then you may choose to use CHROMagar suggested by Gambogou.
You may refer laboratory handbook, "Collins and Lyne's Microbiological Methods"
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