Dear all, I would like to know whether there are any advanced methods of determining chromosome number in plants other than traditional microscopic studies.
Please have a look at this link.
https://www.researchgate.net/publication/43512178_Short_Communication_A_robust_method_for_chromosome_quantification_and_ploidy_determination_in_woody_species
Article Short Communication. A robust method for chromosome quantifi...
Dear Kattula,
Please refer to the following links.
Best regards
SM Najim
http://onion.nmsu.edu/documents/horttech99.pdf
http://www.amjbot.org/content/94/8/1360.full
http://www.caryologia.unifi.it/past_volumes/58_4/58-4_1378.pdf
https://www.researchgate.net/publication/5346638_Chromosome_numbers_in_plant_cytotaxonomy_Concepts_and_implications
Article Chromosome numbers in plant cytotaxonomy: Concepts and implications
Refer Prof.AK Sharma's book of Calcutta University who is well known for his chromsomes reserach.There is wealth of information. Visit Google for moreon Arun Kumar Sharma enowned Plany cytologist and his wife Prof.Archana Sharma. Of course there are sveral links. as given by Dr Nirmala
Hi Katula Zi
Nirmala SVSG in the second number suggestion from the top iz an excellent method. Just identification of a single chromosome through well known chromosome specific DNA sequence of the particular crop species using in situ hybridization the method mentioned. But, simultaneously, you can detect three chromosomes or three arms of the chromosomes by three well known DNA sequences by multi color in situ hybridization iz more powerful. DNA x chromosome hybridization after labeling with isotope or non-isotopic coloring detecting biochemicals such as digoxigenin, biotin are generally used. Please, check next labeling for more than three arms or chromosome identification or numbering. But, special skill obtained through long work and experiences will certainly helpful for this sort of numbering.
thank you very much Dr.Arvind Singh, Dr. Nirmala S.V.S.G, Dr. Salah Mahdi Najim, Dr. Bir Bahadur and Dr. Nav Raj Adhikari for your kind suggestions. Thanks a lot.
Sir, I want to confirm the chromosome numbers in Abelmsochus wild species.
Collect seeds and try if there is no dormancy they will germinate.Harvest the root tips afyter giving colchicine treatment to get good metaphases.Try acetocarmine or acetoorcein, If the species is flowering you collect young buds of different stages in acto-alcohol or any other suitable fixarive and squash anthers and examine.
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