Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 1,913 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View