What are Bacterial counting methods ?

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Using Primers for both classic PCR & Real time PCR?

Hello, Can i use specific Primer for both Classic PCR and Real T PCR ?

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I am looking for journals with speed processing in microbiology fields .?

please can you list them. regards

06 July 2018 10,051 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

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10 August 2024 4,005 2 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

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Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?

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Phil Geis

refer to standard Methods for Examination of Water and Wastewaterhttps://www.standardmethods.org/store/

Sourav Maiti

You can go for MPN. Plating and semiquantitative counting can be done.

this is the classic R2A medium http://aem.asm.org/content/49/1/1.full.pdf

Fateme Taheri

use serial dilution of your sample and then do the pour plate! pour plate should be done with 5 or more plates for each dilution. count the average and CFU per ml. in this method even anaerobic bacteria have a chance to grow.

Muhammad Abdullah Al Mamun

Serial dilution is the simplest way to determine the bacterial count.