What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

How to phrase semi-structured interview questions using IPA without biasing superordinate themes?

Hello, I am preparing a semi-structured interview schedule for an interpretative phenomenological analysis study on marginalized individuals' career experiences as impacted by COVID-19. The aim...

19 February 2021 9,099 2 View

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19 January 2021 6,848 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

How much does radiative heat transfer effect thermal conductivity of materials and how to compare calculations to experirment?

I am calculating the phonon contribution to thermal conductivity for 4 perovskites (BaZrO3, BaSnO3, BaHfO3, and KTaO3) with relatively wide bandgap. When I compare my results for BaZrO3 to the...

27 December 2020 3,232 3 View

Proper method for assessing model invariance following Confirmatory Factor Analysis (CFA) and preceding 2x2 between-subject analysis?

I have produced a good-fitting, reliable baseline model according to CFA. The model contains several factors each loaded by several categorical (ordinal) items. I ultimately intend to analyze the...

15 December 2020 1,668 4 View

How to Reinstall Operating System for a TA Instruments 2910 DSC?

Hard drive crashed on an old windows 2000 that was used to control a TA Instruments 2910 DSC. Installed new hard drive and I am trying to figure out where I can find the software/operating system...

17 November 2020 2,467 3 View

Communities based websites do they serve a purpose?

I'm asking and posing this question as it forms a part of my final project year. I building an ASP.net community-based website coupled with an Oracle database and I'm looking for feedback,...

17 November 2020 7,852 1 View

How can I solve Error: "The coupling service failed to start" during simulation ANSYS 2Way FSI?

Hello, I am dealing with error during lounching 2 Way FSI in ANSYS Workbench. Setup of the Fluent is ok (stand alone I am receiving expected results) and FEM uses Transient Structural. Once I run...

11 November 2020 3,816 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View