I need to use serum from patients as a challenge for primary cells.
However, I am afraid that with heat inactivation of complement I will lose all cytokines and other molecules which make difference between serum from healthy and disease patients.
Do you have any suggestion what can I use instead of heat?
Hello Maja, how are you? Well, do you really need to inactivate the serum for you cell culture? I suggest you first trial with both inactivated and normal serum and see what happen. Some cell lines do not require or do not change the growth or other characteristics of the culture, and also could have some adverse effects. I reccomend the following read. Nevertheless, I am not sure about alternatives for heat inactivation of serum. Hope helped. Good luck.
You can use sterile- filtration method.
Serum, used to supplement cell culture media, is usually sterilized by filtration because heat-treatment may adversely affect its growth-promoting properties. This filter-sterilization procedure is, however, unreliable because viruses and mycoplasmas may easily pass through the filter.
inactivation of complement. The destruction of complement proteins by pathogens (e.g., herpesviruses or measles virus); allergens (e.g., pollens); or in the laboratory
inactivation of complement. The destruction of complement proteins by pathogens (e.g., herpesviruses or measles virus); allergens (e.g., pollens); or in the laboratory,
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