Hello everyone. I have been struggling a lot with my western blots. I am treating my cells with short chain fatty acids and then extracting my proteins using RIPA/protease inhibitor lysis buffer. after that I am qualifying my proteins using Lowry assay, I am trying my best to avoid bubbles and to get accurate results.

the proteins I am trying to detect are vimentin and beta catenin. Migration assays and real time PCRs as well as previous results strongly demonstrate that there will be down-regulation of these proteins in response to the treatment.

when it comes to western I am struggling with equal loading since actin bands are looking inconsistent (especially in the control I am getting thin bands and with other samples as well). and the westerns have not been giving the expected results. I have repeated the westerns many times and I am still struggling... Can someone please help and suggest if there might be anything wrong with the quantification, cell seeding (working on SKOV and PA-1) or loading (although I doubt since previous westerns have given really good results).