My coworkers and I have been trying to screen S. aureus for capsule production using agar plates that have been supplemented with Congo red. My coworker has done this before during graduate school, but at work it doesn't seem to be working. I really want to get these plates up and running, because they'll be useful for some mutagenesis screening we'll be doing in the near future (yes, a PCR will be done eventually on the samples, but assuming I get a large quantity of mutants I want something a bit more economical for the initial screen). We've tried two recipes so far (which are below), and I'm going to buy new Congo Red just to be safe. But does any one have any suggestions to get them working besides that? My group is kind of tapped out on this one.
1) 40g/L Blood agar base, 0.4g/L Congo red, 10g/L glucose.
2) TSA base, 0.8g/L Congo red, 5% sucrose
Hi Kenneth my recipe was BHI agar (52g/L), sucrose (36g/L), congo red dye (0.8g/L). This worked for me every time. Hope it works for you.
Hi Kenneth I have used also the same doses used to Emmanuel and it worked well. You can read the proceedings in this article: http://www.ncbi.nlm.nih.gov/pubmed/17089093..
Hi, in my lab a BHI agar supplemented with sucrose and congo red is used and it works. I coy the link for the paper where the method is explained
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1142068/
Hope it works!
Hi, Had success using agar plates containing 0.8g/l Congo red, 36g/l saccharose in Brain Heart Infusion agar. Congo red we used was fairly ancient but still worked Ok.
Hi, my recipe is similar: Saccharose - 36 g/l, Congo Red (Sigama) 0.8 g/l in TSA (BBL). You can read the proceedings in this article: Freeman D. J., Falkiner F.R., Keane C. T.: New method for detecting slime production by coagulase-negative staphylococci. J. Clin. Pathol. 1989, 42:872-874.
Hello would you please tell how to prepare the congo red concentrated aqueous solution using this receipe : brain heart infusion broth 37 g/L, sucrose 50 g/L, agar 10 g/L and
Congo Red indicator 8 g/L. Congo Red stain was prepared as a concentrated aqueous solution and autoclaved separately from the other medium constituents. Then it was added to the autoclaved brain heart infusion agar with sucrose. Inoculate CRA plates with test organisms and incubate at 37oC for 24 h aerobically. Black
colonies indicate biofilm production.
please read the following link , may help you
http://www.academicjournals.org/article/article1380189385_Mariana%20et%20al.pdf
Peter S Zilm Manel Merradi Martín Fraga
Actually, I want to know what is the basic function they play when we mixed congo red and glucose in nutrient agar plate ?
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