Dear all,
I would like to know whether one has tested the BOS method (plant material dried over CaCl2), a widely used method among plant virologists, for sampling pieces of stem or roots infected by vascular pathogenic bacteria.
THe idea would be to sample pieces (or tranches) of stems in the field, store them in CaCl2 for one/two weeks, and then isolate the bacteria in the lab using an enrichment method (dilaceration of the inner tissues and incubation in a semiselective broth) or simply after maceration in sterile water/tris and plating.
Does anyone have an experience on that ?
Thank you
Emmanuel
Pathogens can colonize all plant organs and tissues. To prevent this, each cell must be capable of autonomously
triggering defence. Therefore, it is generally assumed that primary sensors of the immune system are constitutively
present. One major primary sensor against bacterial infection is the FLAGELLIN SENSING 2 (FLS2) pattern recognition receptor (PRR). To gain insights into its expression pattern, the FLS2 promoter activity in β-glucuronidase (GUS)
reporter lines was monitored. The data show that pFLS2::GUS activity is highest in cells and tissues vulnerable to bacterial entry and colonization, such as stomata, hydathodes, and lateral roots. GUS activity is also high in the vasculature and, by monitoring Ca2+ responses in the vasculature, it was found that this tissue contributes to flg22-induced
Ca2+ burst. The FLS2 promoter is also regulated in a tissue- and cell type-specific manner and is responsive to hormones, damage, and biotic stresses. This results in stimulus-dependent expansion of the FLS2 expression domain. In
summary, a tissue- and cell type-specific map of FLS2 expression has been created correlating with prominent entry
sites and target tissues of plant bacterial pathogens.
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