Dear everyone:
We have an Acquity-UPLC coupled to Synapt g2-si MS system. Recently, the MS was powered off because the electricity supply failed. When I restart the MS, and then run LCMS samples. The LC is not running and only MS can start the scan. On the console window, all of them are in green light. Does anyone have a similar experience and want to share with me how did you fix it?
I've worked on Acquity/MSMS before. For no reason ever should the MS be turned off without venting the vacuum cell. In case of power failure, you should consider providing a high capacity uninterrupted power supply (UPS) that protects your MS. Anyway, after power was restored, you need to turn on all your modules, and from the MS Tune window you need to choose one of the menus in the menu bar (I don't remember which menu is it) and then go to vacuum and then "pump". That should turn the vacuum turbo pump on. In the MS Tune you can see the "Turbo Speed" which must go to 100%. When 100% is achieved, leave the pumping on for at least one night before assuming that the MS is ready.
On the other hand, the UPLC should be ready in a few minutes after powering it on. If you can't control the UPLC from the computer, you may have lost communications, so try to restart your computer and try again. Communication issues are common for instruments controlled by computers, from different manufacturers!
Try these steps and see if you can at least do a solvent prime from the MS Console window.
Note: I assume you have MassLynx as the software.
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