Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Mie scattering of a Gaussian with curved wavefront by a sphere?

Can anyone please suggest some references? I would like to simulate scattering of a fundamental mode of a fiber by a microsphere. I am only familiar with a plane wave case, but in case of a curved...

01 January 2021 1,440 3 View

Blood and tissue DNeasy mini kit 260/230 contamination?

I've been trying to extract DNA from rat liver tissue using Blood and tissue DNeasy mini kit. I followed handbook procedure and all of my samples had low 260/230 (

21 December 2020 7,100 3 View

Student's t-test or DeSeq2 for RNA-Seq data analysis?

Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...

28 November 2020 5,977 3 View

Cre-loxp system does not work (lines #017455 and #017320, JAX). Why?

We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...

16 November 2020 8,701 1 View

Has anybody seen research on Generative Adversarial Networks not image / video / autoencoder based?

Searching for Generative Adversarial Networks applications.

09 October 2020 2,316 2 View

Why after cloning recombinant pET in E. coli DH5a I get a very low plasmid yield after extraction?

I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....

13 September 2020 4,421 3 View

Is there a plausible explanation for the increased frequency of severe hurricanes in the southwest Atlantic Ocean other than climate change?

The Southeastern region of the United States of America, including Florida, Georgia, South Carolina, North Carolina, and the Caribbean archipelagos are being devastated by a higher number of...

16 November 2020 9,463 20 View

How to avoid NaN Value during simulation on Typhoon HIL software?

I have made a system model which is running well in Matlab/Simulink. When same system is implemented using Typhoon Hil software, it is compiled successfully but, showing NaN value (and value is...

02 October 2020 3,282 1 View

How to check previous TC track record from the Japan Meteorological Agency (JMA)?

The TC track record can be checked easily from the Shenzhen Typhoon website, but how to check such records from the JMA website? Looking forward to a positive response.

31 August 2020 6,285 3 View

Is there any well established science that explains the influence of Hurricane forward speed on Storm Surge generation at open coast & bay separately?

Dear Colleagues/Researchers, I'm trying to find out the physics how a fast and slow (separately) moving Hurricane/Tropical Cyclone/Typhoon influence storm surge generation. But so far I haven't...

07 August 2019 7,503 5 View

Is there any well established science that explains the influence of Hurricane forward speed on Storm Surge generation at open coast & bay separately?

Dear Colleagues/Researchers, I'm trying to find out the physics how a fast and slow (separately) moving Hurricane/Tropical Cyclone/Typhoon influence storm surge generation. But so far I haven't...

31 July 2019 7,709 4 View

I was trying to get the wind speed for Hurricane Katrina from reanalysis products but the wind speed is only about 45m/s, why is it very low?

I wanted to observe the rotational velocity of the Hurricane which was reported to be around 175mph, but when I looked at reanlysis products like ERA Interim or 20CRV2C the maximum wind speed I...

24 April 2019 8,262 4 View

I was trying to get the wind speed for Hurricane Katrina from reanalysis products but the wind speed is only about 45m/s, why is it very low?

I wanted to observe the rotational velocity of the Hurricane which was reported to be around 175mph, but when I looked at reanlysis products like ERA Interim or 20CRV2C the maximum wind speed I...

03 April 2019 9,458 5 View

Where can I find sensor data for Hurricane Florence and Hurricane Michael?

I am looking for sensor data (for eg., Satellite, Remote Sensing etc.,) associated with Hurricane Florence and Hurricane Michael? If you have or pointers to it ... Could you share?

04 March 2019 1,576 3 View

Where can I find sensor data for Hurricane Florence and Hurricane Michael?

I am looking for sensor data (for eg., Satellite, Remote Sensing etc.,) associated with Hurricane Florence and Hurricane Michael? If you have or pointers to it ... Could you share?

02 March 2019 2,151 4 View

Climate change, hurricane, rains and fishes?

Hi community, I have been looking for what happens to marine communities during storms and hurricanes and what effects they could have, unfortunately most of the papers focus are about the...

01 February 2019 6,502 14 View