Is it possible to get more than one protein identification from single 2-DE spot. If so, please suggest some papers where these kinds of results are published......
This is described lots of time, i.e.:"Evaluation of two-dimensional gel electrophoresis based proteome analysis technology" Gygi et al. 2000 (PNAS 2000 vol. 97).
We also have a publication about this topic concerning the presence of multiple Proteins in one 2D Spot were we try to give a solution for the accurate quantification of this proteins: "In-Gel 18O Labeling for Improved Identification of Proteins from 2-DE Gel Spots in Comparative Proteomic Experiments" Brödel et al. 2009 (Journal of Proteome Research 2009, 8, 3771–3777 3771)