Trichoderma cultured in broth solution and incubated with higher ph 9.0 but after 15 days of incubation the ph level drastically reduce into 4.5. Please tell me what is the reason
based on the under conditions and a significantly..
Trichoderma spp. are effective biocontrol agents of plant pathogenic fungi and possess varied mechanisms of control. Chemical and physical factors including pH are known to have an influence on these mechanisms as well as on the growth and sporulation of Trichoderma.
The rate of sporulation of the Trichoderma sp. in PDA plates with amended pH values. was also estimated and a significantly high (p≤0.05) rate of sporulation (5×107 spores ml-1 ) was observed at pH 6 after 49 days. The effect of pH on the survival of Trichoderma evaluated using pot experiments showed that the pH values 4.6–5.0 facilitated the highest survival rate of 7.9×105 cfu ml-1 . Effect of Trichoderma sp. on the growth of Fusarium sp. at different pH values was evaluated under greenhouse conditions and a significantly low level of Fusarium colonies were isolated from seedlings treated with Trichoderma at pH 4.6.