How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

How to interpret the following contour plot?

Hi. I am new to Countour plots. I need help with the understanding of the following contour plot. If someone can explain, please.

10 November 2020 3,710 1 View

Qiagen DNeasy vs QIAquick spin column?

Dear Fellows I wanted to confirm if I can use spin columns of QIAquick PCR purification kits for DNA extraction. We finished our DNeasy columns but still have reagents. And we have extra QIAquick...

03 November 2020 6,960 3 View

How to get the DNA sequence/ structure (having specific genes/proteins of interest) for docking?

I am looking forward to explore / validate the role of a drug in breast cancer treatment particularly in the absence of mismatch repair proteins via in-silico analysis. Picking random DNA could...

02 November 2020 408 2 View

Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?

Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?

13 September 2020 7,717 11 View

What are the psychological effects on children during the COVID 19 pandemic?

I am working on the Psychological impact of COVID 19 on children of health care workers. i need references and guidelines for research on this topic.

20 July 2020 2,791 19 View

How to find Slow and Fast light?

I am facing a problem to calculate the Slow and Fast light using Matlab. Can some share a simple code for Slow and Fast light. Thanks Xie Xie Danke.

13 June 2020 3,955 2 View

Which modelling technique to predict corona virus trends in future?

Hi, I am working on time-series data for the last 2 years. However, I have never used forecasting models. I want to enhance my statistical skills. As corona is all around us, I was thinking to...

29 March 2020 7,452 6 View

What would be standard formula/equation to derive post-test mean & standard deviation values from the established study for meta-analysis?

Since in some studies, authors only report the mean difference and mean difference standard deviation. Kindly, help me out to get the absolute mean and SD of post-test measures. Thanks

22 March 2020 5,358 7 View

How to calculate percentage inhibition in ABTS method?

27 January 2020 2,632 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View