05 August 2019 0 8K Report

Hi, I have been trying to delete the gene sifA from Salmonella Typhimurium and keep failing. I have successfully transformed the pkd46 plasmid to my target strain but I keep failing to transform the linear DNA which I have amplified from pKD3. when I do gel purification for the PCR product obtained from pKD3 I'm getting very low levels of DNA concentrations (less than 20ng). I have induced my target strain containing pKD46 with 10mM arabinose. For transformation, I have applied an electric field from 2.0kV-2.5kV. Can you please help me to solve this problem. Thank you.

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