Hello there!
I'm trying to analyse phenolic compounds (phenolic acid and flavonoids) in quinoa seeds by HPLC but I'm having some problems with the hydrolisis of bounded compounds. I would like to ask, in your experience, which is the best sample treatment method in order to analyse total (free and bounded) phenolic compounds.
I have read that acid and basic digestion is very common, but I think your experience would be a good point to start with.
Thank you for your attention. I'm looking forward to your reply.
Carlos Abc " I know there are many articles available but I'm running out of time "
Nobody is expected to work for you. Personally, I'm always running of time.
Mr Geletti excuse me if my post sound kind of rude. My apologies.
What I'm trying to say is there are many articles (of course I have read them even tried many of them), but in the field of phenolic compound analysis, results may vary depending on the methodology used.
The purpose of my post was to ask for a recommendation. Mr Geletti, if you have tried any methodology with good results you may help me with your experience and recommend me any research article in order to test it.
In any case, thank you very much for your attention.
Have a good day.
Yes, " I'm running out of time " does not sound well.
Yes, " in the field of phenolic compound analysis, results may vary depending on the methodology used." Therefore, there is no the best analysis. The best is the one that you can perform in your lab and which you understand well.
This is the review with the full text
Article Techniques for Analysis of Plant Phenolic Compounds
Are you sure you want to quantify only phenolics? You might need to determine the total content of antioxidants?
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