.…
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Need to check quorum sensing activity of Vibrio campbellii.
20 January 2021 570 5 View
I have some Vibrio isolates from Brackishwater fish and environment. I am unable to identify two of them unto species level. It is showing conflict between Biochemical identification and Molecular...
03 January 2021 691 7 View
In our research, the DNA vaccine encoding Hsp33 gene of Vibrio anguillarum was made and proved to confer immune protective effect for fish. however, the Hsp33 was belong to the intracellular...
08 September 2019 1,209 5 View
01 September 2019 9,006 4 View
I have tried to do the gene knockout using the same plasmid in organisms like Vibrio cholera and Shigella flexnerii and failed. It will be very helpful if you can suggest a good protocol for...
10 November 2018 2,049 4 View
I'm currently doing PCR for my study. I used heterologous internal control which I need to include plasmid (Vibrio Cholerae HemM Gene) in each samples. But after I've run about 2 to 3 times pcr,...
10 November 2018 9,443 7 View
06 November 2018 6,967 6 View
When I image my biofilm, it seems that dispersal has taken place. I grow the biofilm in LB media for 24 hours. I wonder if it is because of my washing steps using PBS. Should I mabye use LB...
07 August 2018 8,614 5 View
I'm using SYBR iQ supermix and the vvha primers described here: Real-Time PCR Analysis of Vibrio vulnificus from Oysters I'm not sure if there is an issue because these primers are for a probe...
07 August 2018 8,596 2 View
