Hi all, I am a PhD student working on the effect of Ang (1-7) on Alzheimer's disease. I am using Ang (1-7) peptide in electrophysiology and Western blot techniques. I could not see any effect with this peptide. More likely something related to the preparation!!

This is the one that I am using. https://www.apexbt.com/angiotensin-1-7.html#protocol

I prepare it in a distilled water ( Mass: 25 mg

Molecular weight: 899 Daltons

Concentration: 25 mM

Volume of water= 1.112 ml

And allocate them in 20ul tubes.)

Any tips to make the peptide work please !!!

Thanks a lot