Hi. I need to do FACS for three subsets of thymic DCs and am looking for a thymus enzymatic digestion protocol. Appreciate your recommendations. Thank you!
I am trying to run an ordinal logistic regression using Generalised Linear Model from SPSS. There variables are A,B,C,D, and a moderation variable A*C. Variables A and D are continuous variables...
02 December 2020 5,159 3 View
I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...
01 December 2020 4,632 3 View
Hello, I have recently stumbled upon a couple of articles consisting of 'case-based review' as the title of their publication. May I ask how different is a case-based review compared to a case...
29 November 2020 8,732 2 View
I am trying to simulate the behaviour of a Piezoelectric sensor, positioned behind a plate subjected to ad impact and consequential oscillations. I have set a transient analysis and associate the...
17 November 2020 3,377 3 View
I am trying to model a piezoelectric sensor attached to an aluminum thin plate. I know that it is possible to model a PZT using Mechanical APDL, selecting the proper elements, such as PLANE13,...
13 October 2020 7,915 11 View
I conducted an analysis in autodyn saving at gauges points the displacements along z. Now, I would need to use this output data as input for analysis in the static structural environment. Since I...
15 September 2020 5,201 3 View
I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?
23 June 2020 2,115 5 View
the lithium-ion batteries (graphite||LiCoO2) charge at 500mA to 4.2 V and pulse disharge at 3A (discharge 5S, then rest 10S )to 3V. The curves (capcity retention vs.cycle number) is step -like....
02 January 2020 8,823 1 View
As we know, due to the tendency to decrease the overall Gibbs free energy, grain boundaries in crystalline materials are subjected to thermal-induced migration, either through a diffusive flux of...
23 December 2019 3,272 2 View
I found contradictory information about this genus and I would like to understand its correct geographical range.
13 December 2019 8,348 8 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...
18 February 2021 3,453 4 View
Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...
17 February 2021 2,536 3 View
Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...
16 February 2021 1,664 3 View
I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...
09 February 2021 1,293 1 View
Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...
09 February 2021 3,383 4 View
I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...
08 February 2021 733 6 View