Hi. I need to do FACS for three subsets of thymic DCs and am looking for a thymus enzymatic digestion protocol. Appreciate your recommendations. Thank you!
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
21 July 2024 4,676 4 View
My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...
27 May 2024 7,529 2 View
I tested DNA structure (5uM dissolved in 50 mM Tris) using JASCO-1500 but found no peak at wavelength 220-350. The results look like a wave shape throughout the wavelength...
14 April 2024 4,003 2 View
For my project I have created an intervention that involves educating patients about a topic. I make them do a survey before, put up my intervention, then do the same survey after. I want to...
22 February 2024 6,080 2 View
Hi: I am performing immunofluorescence staining on 100 um 4%PFA fixed pancreas sections using our self-made polyclonal primary antibody. Tissue was gradient dehydrated in methanol diluted in 0.2%...
28 January 2024 867 3 View
Hello, I am currently attempting to optimize a protocol for extracting microbial DNA from cotton swabs that were swabbed against fish skin. I am wondering if you have any recommendations for the...
24 January 2024 6,175 0 View
We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...
04 January 2024 6,926 0 View
17th refers to the experimental parameter code Material is mesoporous silica nanoparticles (MSN-NH2). Use deionized water as dispersant and ultrasonic vibration. The theoretical concentration is...
02 January 2024 4,160 3 View
In our study, each participant rated several organizations randomly selected from a big pool on some attributes (denoted as) x1, x2, and x3 and an outcome y, and each organization has multiple...
14 November 2023 2,756 5 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...
20 July 2024 3,392 2 View
Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...
18 July 2024 5,225 3 View
One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...
18 July 2024 9,907 12 View
How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...
17 July 2024 3,255 4 View
Does anyone have any case studies on the Environmental Impact of Organic Manure Production Unit? Environmental Impact of a Organic Manure Production Unit Facility is what I studied but...
16 July 2024 5,400 6 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View