This is very important area for the community of the world, what i want to ask is is there any means or approach how i am part of this project?

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How can I upload Climpact2 tool into R software?

Dear all, I want to determine climate extreme indicators using "CLIMPACT2" tools in R software. However, I am facing some difficulties with the installation process. When I try to run the code, I...

15 April 2024 7,625 1 View

How can I use a General Regression Neural Network in JMP to include climatic data into an allomtric equation to compute Coffee's aboveground biomass ?

I want to estimate the aboveground biomass of Arbica coffee under present and future climate change using the SAS-JMP application's General Regression neural network (GRNN). How can I use a...

24 October 2021 9,506 2 View

Is there anyone who can help me how to install GRNN package and execute the R model?

I want to use the General regression neural network(GRNN) model in r-software to simulate the reactions of aboveground biomasses of perennial trees to climate change factors (rainfall, minimum and...

01 September 2021 3,510 4 View

How can I download data in bil format from WorlClim version 2? Or How can I convert Bioclim data in GeoTIFF to Generic grid (Bil) format?

I want to download BioClim data containing the Generic grid format (bill) to use in species distribution modeling

23 July 2020 864 1 View

Is there any method of calculating crop water requirement for plant having 0.94 crop coffecient and growing under 50% shade cover?

I want to determine crop water requirement for coffee plant s under different shade cover and I want to understand the effects of shade cover on crop water requires

13 February 2020 5,149 4 View

How can I simulate above ground biomass of coffee plant under climate change scenarios?

Dear Sir, thank for your contribution to help me I want to simulate Coffee above ground biomass dynamics over the next century. I have determined the current above ground biomass of the given...

31 January 2020 5,994 0 View

How can I estimate the future shade cover under climate change scenarios ?

Hello everyone, I want to explore climate change adaptation option through improving the tree shade cover level for coffee plants. The current values of optimal shade cover at higher plant...

28 January 2020 9,184 4 View

What is the physical meaning of increasing or decreasing the formation energy with the number of doped N atom in graphene??

What is the physical meaning of increasing or decreasing the formation energy with the number of doped N atom in graphene?? And how do we relate this with the increasing of more active sites with...

28 January 2020 581 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,032 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,784 9 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,254 3 View