Smart VacPrep, anyone had problems with degassing?

12 October 2020 1,907 2 View

Anyone know an effecient method for transfection / siRNA knockdown in organ culture (retina)?

Hello everyone, I have made a nice protocol for culturing whole adult rat retinas in vitro. This is also called organ cultures or whole mount cultures. The retinas are sitting on a filter in a...

15 July 2020 5,300 1 View

Factors affecting precipitation of serum proteins?

Hello, In most modern protocols for metabolomics/lipidomics proteins are precipitated using solvents such as methanol, acetonitrile or isopropanol. After vortexing/mixing the next step is often...

28 April 2020 3,383 2 View

Protocol for FTIR analysis of activated carbons?

Want to analyse functional groups on activated carbons (biomass based). Is there a good protocol including sample preparation and instrumental setup?

02 March 2020 6,972 2 View

Personality. Enherited or socially constructed?

What is your take?

28 July 2019 7,443 17 View

DNA barcoding: is there a COI alignment that spans the Eukaryota?

Regarding the DNA barcode COI (cytochrome oxidase subunit 1), I'm looking for a multiple sequence alignment that, ideally, spans the Eukaryota. Is there any such alignment available for...

30 September 2017 506 5 View

How can you get rid of air bubbles (or blisters) on butyl compound used for inner tubes?

Looking for a method to eliminate air bubbles on butyl inner tube? there are blisters on cured butyl inner tubes, is this a compounding problem or machinery process?

16 September 2016 5,717 2 View

Should I use "locus", "gene", or "marker"?

20 March 2014 8,234 4 View

International business, further globalisation or backlash?

In the field of international business the trend has been a move towards globalisation and integration. However research has shown that lately most government policies have been of a protectionist...

01 January 1970 8,882 59 View

Ambivalence: Where to draw the line between the pathological and the non pathological

When it comes to Ambivalence, it can be a symptom of mental illness but it is also something we experience in our everyday life ( I certainly do, and my wife hates it) . Where would you draw the...

01 January 1970 6,670 9 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View