It's about quinine (alkaloids) andfluorescence test
The fluorescence test depends on the wavelength of the radiation used. what is the maximum one length you are using?
Please specify the wavelength value used!
Fluorescence test: Solution of the alkaloid in oxygenated acids (e.g H2SO4, HNO3 or phosphoric acid) to give blue fluorescence (+ ve with quinine and quinidine).
Try adding dil. H2SO4
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