Greetings to all,
May someone list out techniques or methods which can be use to determine the type of compound present in an extract of plant?
It will be better if the technique does not require an additional reagent for analyzing process such as HPLC.
Something like FTIR is good. Does not depend on reagent for identification process.
MS, FTIR, NMR, UV.... However, this assumes you have a 'pure' compound, thus HPLC or GC.
Any plant extract will contain a complex mixture of compounds, so normally the best methods for identifying individual compounds is to use a separation technique such as chromatography (GC, LC, HPLC etc) to separate the mixture and mass spectral detection to help identify the compounds. FTIR is often not good for individual compounds as it detects groups of compounds by detecting their functional groups and the absorptions are very similar for all compounds in that group. Sometimes it is useful for bulk materials or compounds which have very characteristic absorptions (such as beeswax) but you already know that this is a plant extract.
There is a lot of papers on GC-MS analysis of plant extracts - mostly after derivatization of specific "target" groups of compounds.
You can make also HPLC screening for example in reversed phases with UV-DAD detector. On the basis of spectra in UV you can say something about your compounds - for example you can look for quinones. Using amperometric detector you can look for anitoxidants. The literature is very rich.
regards,
G
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