Hi, friends, we met a technique problem recently, is that, when 3T3-L1 cells (P6-P10) were induced for adipogenesis in 12-well plat, at D3-D5, the cells are so easy to fall off and flaoted later. So pls let me know how you solved such problem? We used DEME/H, 10% FBS and cocktail inducer. How about if we coat the plat with something?
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