Hi,

Did you find out? I m interested in the answer ;)

Hi... Pauline Bourbon... not yet.. i still don't have an answer... one of the journal (J Infect Chemother ) reviewer asked to calculate the FIC. Now the paper is rejected since i didnt have any proper answer.

hi, did you find your way out?

FIC index calculation confuses me too.

looking at protocol 5 in this document, i still cant figure it. please help if you now know how.

Basic microbiological techniques used in antibacterial drug discovery

https://www.ncbi.nlm.nih.gov/pubmed/21953398

https://www.ncbi.nlm.nih.gov/pubmed/24920779

Hello. In case you still need this information. A couple of papers helped me figuring out how to calculate the FICs from a checkerboard. I used the data given from one of the examples and was able to replicate the FIC calculations. Here are the references.

Hsieh, M. H., Yu, C. M., Yu, V. L., & Chow, J. W. (1993). Synergy assessed by checkerboard a critical analysis. Diagnostic Microbiology and Infectious Disease, 16(4), 343–349. https://doi.org/10.1016/0732-8893(93)90087-N

Meletiadis, J., Pournaras, S., Roilides, E., & Walsh, T. J. (2010). Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, Monte Carlo simulation analysis, and in vitro-in vivo correlation data for antifungal drug combinations against Aspergillus fumi. Antimicrobial Agents and Chemotherapy, 54(2), 602–609. https://doi.org/10.1128/AAC.00999-09

Dear Rony Fernando Orobio Hurtado.

Thank you very much for your detailed information.

Satish

Dear Epameinondas Angelopoulos

The paper you shared was helpful.

There are several ways to compute FIC. This paper may shed more light: Comparison of methods of interpretation of checkerboard synergy testing by Bonapace et al., 2002