IS there a specific procedure to thaw cells from liquid nitrogen?

Hi all! I have been thawing a few cell line stocks we had kept in liquid nitrogen and faced a couple of problems not being able to get the cells adhere to the T25 flask easily. I am not sure if...

27 December 2020 5,525 2 View

How can we split cells from T25 to T75 flasks?

Hi all, I am trying to wrap my head around splitting cells into different surface-area flasks. I could really use your help! I currently have 8 T-25 flasks of HEK-293T cells. I am familiar with...

04 November 2020 3,023 4 View

Can I use low concentrate Sulfuric acid into drip irrigation?

Generally water alkalinity to clot the dripper nozzle due to salt formation. So bring down the irrigation water pH to 5.5 to 6.5 may useful for prevent for salt formation as well as crop...

31 August 2020 675 3 View

How do I solve this error when trying to execute the GROMACS command "xpm2ps"?

Hi all, I hope you could help me out with this question in GROMACS. I am trying to use the "xpm2ps" command in GROMACS to output a postscript file from an .xpm file. However, I keep on getting...

15 April 2020 6,028 2 View

What is the best method for measuring copper accumulation in bacteria and fungus?

I am doing research on microbial remediation. How to measure the copper accumulation in bacteria and fungus ? The supernatant can be measured by AAS or ICP-OES OR Acid digestion of bacterial...

16 December 2017 4,447 3 View

Does further heating process make any structural variation in metal ferrite sample ?

05 October 2017 5,675 3 View

How do we calculate wt% for composites ?

i want to make composite with two different oxide materials in review papers one material weight is fixed in gram another material was added to different wt% so i don't know how to calculate the...

04 October 2017 3,405 5 View

Hello, how do I interpret the below confocal images?

I recently did IHC staining of mouse brain tissues with four different primary antibodies, but am unsure as to how I am supposed to assess and troubleshoot these confocal images. The following...

28 September 2017 9,502 3 View

Is there anyone to support our project Bio gas from brown seaweed ... (Looking for a partner or a collaborative agent or a financial support)?

15 July 2016 6,007 3 View

What is the best method to concentrate a large volume of protein samples?

05 August 2014 2,219 5 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View