PFA Fixed Brain Tissue is brittle when I section. How Do I rescue this?

I have rat brain samples, that I fixed with 4% PFA but on sectioning they come out very brittle and fall apart. I stopped sectioning because of this. I am looking for a way to recuse the rest of...

19 October 2020 2,488 4 View

Any research topic ideas on AI and Project Management ?

I am looking to conduct my thesis research on the use of Artificial Intelligence in Project Management and would welcome topic Ideas Thanks N

29 August 2020 8,693 11 View

How do I decision between innovative outlier and additive outlier models?

I am working on a time series data and I want to determine the order of integration for the various variables. When looking at the graph I observed a potential break point in the some series...so...

18 June 2018 6,401 2 View

What is the best statistical approach to determining socio economic status of a population with a certain disease?

I will appreciate a quick statistical advice...

11 October 2017 7,761 3 View

What are the different carbon sources for alpha amylase production?

Am trying to work on this topic 'effect of pH, temperature and metal salts on the stability and activity of alpha amylase produced from Rhizomucor pusllus'. Just need a carbon source for the...

02 August 2015 5,512 3 View

Suggestion on Research gaps in microbiology of biogas production and optimization. Ideas, suggestions and links to relevant works are welcome

01 January 1970 7,089 5 View

Reducing or Increasing flow rate without temperature change.

If i have water at 90 C, (pressure 16 bar) with a flow rate of 80 kg/s and i need to reduce the flow rate to 20 kg/s before running it through a heat exchanger, can i achieve that with a flow...

01 January 1970 2,721 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View