Submandibular bleeding

More Simon Kalita-de Croft's questions See All

What is the best way to analyse dual recordings in order to find evidence for a monosynaptic connection?

I have dual recordings of neurons, in which one cell was stimulated to fire an action potential, and I record synaptic currents in the other cell. This gets repeated 30 times for each direction in...

08 September 2013 5,472 10 View

Has anyone got experience using the GCaMP3 mouse model for in vivo imaging? If so, how good is the fluorescent signal?

I'm looking at using a GCaMP3 mouse line to do in vivo imaging of hypothalamus in a mouse, do you guys think that the signal will be bright enough ?

08 September 2012 838 3 View

Submandibular bleeding

I was wondering if anyone uses the submandibular bleeding technique for repeated blood sampling from mice. If so, How many samples can you acquire? and of what volumes? Also, what does the health...

01 February 2012 2,797 9 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

What is the difference between neuropsychology and cognitive neuroscience ?

The main differences

14 July 2024 3,158 4 View

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

06 June 2024 2,234 1 View

Internationally recognised publishing outlets specialised in publishing science-based futuristic papers?

Does anyone use or know of any internationally recognised and reliable publishing outlet (i.e. Journals) that are specialised for submission of "futuristic" articles which are based on the...

28 May 2024 2,946 2 View

How can we effectively model RPE signals at the single-trial level in EEG data?How can researchers disentangle overlapping ERP components effectively?

Dear all, I need your expertise on this matter. I have an article under review and I got some comments that I need your help with: In my research, I investigated the FRN (200_300ms post_feedback...

23 May 2024 4,953 0 View

Can anyone explain why my EGFP expression increased (but Ai9 RFP decreased -like normal) after imaging with a confocal microscope?

I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...

23 May 2024 7,776 2 View

Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

12 May 2024 4,614 3 View

Should a biphasic electrical stimulation pulse on neural networks be positive followed by a negative phase or negative followed by positive phase?

I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...

09 May 2024 7,167 1 View