Hello everyone.
The aim of my current project is the identification of novel therapeutics for Diabetic Retinopathy. We are using the STZ rat model for the induction of Diabetes, using a single dose of 70mg/kg for each animal. Our problem is that four weeks after the STZ injection -and although all the STZ-treated animals display high glucose levels and polyuria as symptoms, we do not observe any reduction in the number of retinal cells (e.g. amacrine cells). Does anyone have any idea about the cause of these observations?
Thank you for your time!
Recently we performed an experiment on a rat model of diabetes induced with STZ. We have the similar results in diabetic group compared to control with respect to apoptosis. We thought that the duration (30 days in our experiment)after generation of DM model is not enough to observe. So we concluded that the time period might be longer to observe the loss of cells in the retina.
Dear Stavroula
Diabetic retinopathy is a chronic process induced by chronic hyperglycemia in STZ rat model.
We have investigated similar study recently. I suggest you read our article entitled : ‘ Chronic administration of Rosa canina hydro-alcoholic extract ...’
so it takes at least one month to induce retinopathy in STZ rat model
I hope you follow me to read my new articles
thanks
Every rat strain reacts differently to the STZ. The SD rat develops only a mild retinopathy, whereas the Wistar rat shows high levels of retinal inflammation. Apoptosis is not a very good marker for retinopathy, especially when establishing a new model in your lab. The earliest observable changes are loss of inner retinal function in electroretinography and pericyte loss. If you are interested in the necessary protocols and references I'll gladly send them to you.
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