I am currently trying to characterize shortmers of large nucleotides (up to 10k bases) from HPLC fractions and the samples I have available are of very low concentration, especially after fraction collection. Does anyone have experience in this area and have suggestions for how to concentrate the target while removing the mobile phase reagents in the matrix?
I've tried rotovaping followed by purification by silica spin columns with mixed results and concentrations that are still quite low. I've considered TFF spin columns but those are MWC and I need to retain all shortmers and demonstrate that they are indeed nucleotides.
To add to Guobing Xiang 's answer, I'm assuming you use reverse phase HPLC to purify your samples?
Although this is written for flash chromatography, the same general procedure can be used for solid phase extraction cartridges or a small preparative HPLC column. I recommend an AQ type C18 to avoid phase collapse:
https://www.teledyneisco.com/en-us/Chromatography_/Chromatography%20Documents/Application%20Notes/Desalting%20Samples%20with%20RediSep%20Gold%20C18Aq%20Columns%20App%20Note.pdf
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